Fitc rabbit anti- active caspase-3

Author: opxd

August undefined, 2024

WebAug 20, 2013 · The caspase activity was assessed by the FITC rabbit anti-active caspase-3 (BD Pharmingen, San Diego, CA, USA). The cells were treated with CuE of 0, 1.25, 2.5 and 5 μM for 24 h. The caspase activity was detected and inspected by the FACSCalibur flow cytometry. Data was analyzed using WinMDI 2.8 free software (BD, … WebTNF-alpha is synthesized as a 233 amino acid (aa) transmembrane protein (mTNF-alpha) with a theoretical molecular weight (MW) of 26 kDa (1,2) that forms a homotrimer. mTNF-alpha is cleaved by TNF-alpha converting enzyme (TACE) and released in its 157 aa, 17 kDa soluble form (sTNF-alpha) (1-5). Both mTNF-alpha and sTNF-alpha are capable of ...

Anti-Caspase3 (active form) Antibody, clone 3D9.3 - Sigma-Aldrich

WebCells were stained with FITC rabbit anti-active caspase-3. A–F are the representative caspase 3 histograms of the PPD-treated cells. Percentage of caspase 3 was calculated and presented in G. WebThese antibodies target Caspase 3 in Human, Mouse, Rat, Non-human primate and Goat samples. Our Caspase 3 polyclonal, monoclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Cell treatment, Knockout and Knockdown to confirm specificity to … note taking format

cleaved-Caspase 3 antibody (25546-1-AP)

WebSpecificity. Caspase-3 is a member of the interleukin-1 β-converting enzyme family. Caspase-3 is thought to be associated with induction of apoptosis. Caspase-3 is … Web别名 A1 catalytic; A2 catalytic; Alkali myosin light chain 1; Alkali myosin light chain 3; C36E6.3; CMH10; DKFZp779C0562; KMLC; MLC 2; MLC 2v; MLC; MLC1F; MLC2; MLC3F; C-type lectin domain family 5 member A; C type lectin domain family 5 member A; C-type lectin superfamily member 5; CLEC5 A; CLEC5A; CLECSF 5; CLECSF5; MDL 1; MDL-1; … WebRabbit Anti Human Active Caspase 3 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ... FITC-conjugated goat anti-rabbit and Texas Red-conjugated horse anti-mouse IgGs from Vector (Burlingame, CA, USA); HRP-conjugated donkey anti-rabbit and … how to set hibernate windows 11

Flow Cytometric Detection of Activated Caspase-3

Caspase-3 Antibodies - Novus Biologicals

WebCaspase-3 Antibodies. Caspase-3, a key mediator of apoptosis, requires induction for activation and PARP cleavage. We offer antibodies to detect the pro and active forms of … WebWestern blot - Active Caspase-3 Rabbit mAb (A11021) Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells … how to set hibernate option in windows 10WebJan 9, 2024 · Caspase-3 expression in HCT-116 cells was quantified through flow cytometry by using the FITC rabbit anti-active caspase-3 IgG antibody. A significant increase in the caspase-3 FITC-MFI was observed in HCT-116 cells treated with AgNPs (1936, 1.5-fold increase) and those treated with cisplatin (44 μg/mL) (4551, 4.5-fold increase), as … note taking formats for middle school

"Web25546-1-AP targets cleaved-Caspase 3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. Stable for … " - Fitc rabbit anti- active caspase-3

Fitc rabbit anti- active caspase-3

Anti-Caspase 3 Antibody Products Biocompare

Webanti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody II. Warnings and Precautions 1. For Research Use Only. Not for use in diagnostic procedures. 2. Reagents contains sodium azide; harmful if swallowed or absorbed through the skin. Sodium azide can WebCellular detection of the apoptotic markers caspase-3, bcl-2 and Bax activity was determined by FCM employing the fluorescein isothiocyanate conjugated monoclonal antibodies (caspase 3 FITC, Rabbit anti- active caspase 3 cat. No, 559341), (PE Hamster Anti-Mouse Bcl-2 Set Cat No. 556537) and (Bax Monoclonal Antibody (6A7) Cat No. …

Did you know?

WebDec 1, 2024 · To identify whether compound 1g exhibits apoptosis, we performed apoptosis assay using Annexin V/FITC, and the fluorescence intensities of FITC-conjugated annexin-V and PI in cells and were analyzed using flow cytometry ().Apoptosis is the critical process for the death of the cancer cells. Upon exposure with compound 1g at IC 50 value, 44.34% … http://pufei.com/static/product/bd/550/1505231617e995b8.pdf

WebFITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission … WebFeb 1, 2008 · USA: rabbit anti-active caspase-3 polyclonal antibody (cat. no. 557035), FITC- conjugated rabbit anti-active caspase-3 monoclonal antibody (cat. no. 559341), and PE-conjugated affinity purified ...

WebMay 3, 2000 · The presence of a low amount of active caspase 3 in the treated viable cells was confirmed in situ with PE-conjugated anti–active caspase 3 antibodies. The same antibody was used in combination with FITC-annexin V and CD45–PC5 to study caspase 3 activation in acute leukemia blast cells after in vitro DNR and IDA treatment. WebActive caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This …

WebThis Caspase-3 Antibody (31A1067) - (Pro and Active) was developed against full-length recombinant human caspase-3 protein. The antibody detects both pro Caspase-3 (~32 …

WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042) Research with confidence – consistent and reproducible results with every batch. Long-term and … how to set hibernation in windows 11WebAnti-Caspase3 (active form), clone 3D9.3 is an antibody targeting the Caspase3 (active form) protein, validated for use in WB, ICC & Flow. Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Caspase3 (active form) in Hela and Hela/staurosporine 3 hr cells. Flow Cytometry Analysis: A 1:400 dilution from a ... note taking free onlineWebCaspases play a crucial role in cell apoptosis or programmed cell death. 70 Effector caspase 3 is responsible for the actual cleavage of cellular components during apoptosis. Hence, in the present study, caspase-3 level was estimated using FITC rabbit anti-active caspase-3 IgG antibody assay kit (BD Biosciences, catalogue no. 559341). note taking formats for collegeWebWestern blot - Active Caspase-3 Rabbit mAb (A11021) Western blot analysis of extracts of Jukat cells, using Active Caspase-3 Rabbit mAb (A11021) at 1:500 dilution.Jurkat cells were treated by Staurosporine (1uM) at room temperature for 3 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. how to set hibernate in windows 11WebCells were subsequently stained with the FITC rabbit anti- active caspase-3 antibody (clone C92-605). Cells were then washed and resuspended in BD Perm/Wash™ buffer … note taking free programsWebAbcam fitc rabbit anti active caspase 3 Fitc Rabbit Anti Active Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more how to set hibernation mode windows 10WebAug 17, 2024 · Plants are a versatile source of biologically active compounds for anticancer drug discovery ... Primary and secondary antibodies included: rabbit anti-Bcl-2, rabbit anti-poly-ADP-ribose polymerase (PARP), rabbit anti-caspase 3, rabbit anti-AMPK, rabbit anti-mTOR, rabbit anti-Bax, mouse anti-tubulin, anti-rabbit and anti-mouse IgG HRP … note taking format template