CT-GFP Fusion Expression kit contain two boxes: 1. TOPO™ cloning box with pcDNA™3.1⁄CT-GFP TOPO™ cloning vector, dNTPs, salt solution, control PCR template and primers, forward and reverse primers for sequencing and PCR screening, and a GFP expression control plasmid. Store at -20°C. WebApr 11, 2016 · We transfected PC12M cells with full-length APP-GFP, C99-GFP (β-CTF), C83-GFP (α-CTF), or AICD-GFP and examined endosomes by live cell imaging (Supplemental Figure 1B). Bright foci of GFP + intracellular structures were present in PC12M cells that overexpressed APP-GFP or C99-GFP. In contrast, cells expressing …

Sequential Proteolytic Processing of the Triggering ... - ScienceDirect

WebAug 27, 2013 · Having established AP2 as a bridging molecule to mediate the interaction between LC3 and APP-CTF by IP, we next aimed to understand how the interaction of LC3 with AP2M1 and PICALM was affected by starvation-induced autophagy. HeLa cells were analyzed by IP experiments using anti-GFP antibody. WebFeb 27, 2024 · We overexpressed GFP or GFP-tagged TSPN6 in MCF-7 cells and performed anti-GFP immunoprecipitation, followed by Western blot analysis of the precipitate (Fig. 3 B–D). We used two alternative … true wax

CLU co-localizes with cytoplasmic TDP-43 inclusions in ER stressed ...

[email protected] 646-738-8574 The Neurofibromatosis Clinic Network (NFCN) was established by the Children’s Tumor Foundation in 2007 to standardize and raise the … WebMay 1, 2024 · CTFs with GFP tag were used for visualization and tracking studies and non-GFP-tagged CTF cells were used for viability studies. Mouse osteoblasts (MC3T3) This cell line from mouse calvaria was acquired from the European Collection of Authenticated Cell Cultures (ECACC, UK). These cells are osteogenic precursor cells that can differentiate … WebSep 1, 2013 · The β-CTF-IRES-GFP retrovirus rendered low fluorescence intensity that was insufficient for dendritic measurements. To solve that problem, dendritic trees of β-CTF expressing neurons were characterized after coinfection with two retroviruses carrying β-CTF-IRES-GFP and CAG-RFP (Fig. 5). Dendritic length was then measured from … true wash poplin shirt with stretch