Camptothecin stock solution
WebApr 14, 2024 · VIQ Solutions is a global provider of secure, AI-driven, digital voice and video capture technology and transcription services. VIQ offers a seamless, comprehensive solution suite that delivers intelligent automation, enhanced with human review, to drive transformation in the way content is captured, secured, and repurposed into actionable ... WebJul 10, 2024 · First , I take 2mg of CPT dissolved in 1ml of DMSO as stock solution, so …
Camptothecin stock solution
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WebCamptothecin sodium selectively inhibits the nuclear enzyme DNA Topoisomerase, Type I. Several semisynthetic analogs of camptothecin have demonstrated antitumor activity. ... Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months). HS Tariff Code: 2934.99.9001 Instruction: View handling instruction ... WebCamptothecin is dissolved in DMSO at 10 mg/mL and serially diluted in 96-well microtiter plates to which the 32P end-labeled pBR322 DNA and topoisomerase enzyme are added. ... What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol? Enter 197.13 into ...
WebAug 6, 2013 · Abstract. UV–Vis spectra of camptothecin (CPT) in toluene, acetone, ethanol, acetonitrile, dimethylsulfoxide and aqueous solutions are investigated by UV–Vis spectroscopy and DFT calculations. The structures of CPT and its π-dimer have been optimized using B3LYP and B97D functionals with cc-pVDZ basis sets. Web1.1 Each vial of DMSO stock solution (100 µL, 2 mg/mL, 3 mM) should be used only once. Any unused vials should be stored at < -20℃. ... Jurkat cells can be treated with camptothecin for 4-6 hours) to induce apoptosis. 3.2 Centrifuge the cells to get 1-5 x 105 cells per tube. 3.3 Resuspend cells in 500 µL of JC-10 working solution (from Step ...
WebCamptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulates hypoxia-inducible factor-1α (HIF-1α) activity by changing microRNAs (miRNA) expression … Webcamptothecin peptide. Antibody–drug conjugates (ADCs) are composed of a monoclonal …
WebCamptothecin (NSC-100880, CPT, Campathecin, (S)- (+)-Camptothecin) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Camptothecin induces apoptosis in cancer cells via …
Web1. Prepare Camptothecin stock solution (Sigma-Aldrich Cat. No. C-9911): 1 mM in … dance by lee ann womackWeb2 days ago · The Camptothecin Market report is a comprehensive document that … birds that owls eatWebPreparing Stock Solutions for Camptothecin. The following data is based on the product molecular weight 348.36. Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to … Camptothecin is a natural product inhibitor of TOP1 that has led to the discovery of … birds that peck on metalWebWherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. ... (camptothecin) in DMSO, fixed with 4% formaldehyde for 10 minutes ... dance by paul haywardWeb( S )- (+)-Camptothecin binds irreversibly to the DNA-topoisomerase I complex, inhibiting the reassociation of DNA after cleavage by topoisomerase I and traps the enzyme in a covalent linkage with DNA. birds that peck on housesWebIsomerases. DNA Topoisomerases. Keywords: CPT 11, CPT 11 supplier, DNA, topoisomerase, I, inhibitors, inhibits, antitumor, Isomerases, … birds that peck treesWebCamptothecin is a cytotoxic plant alkaloid with antitumor properties; prototypic DNA topoisomerase I inhibitor. Induces single strand DNA breaks and protein-DNA crosslinks. ... *When preparing stock solutions always … dance by rdi